Investigating the Use of Prenatal Cell-Free DNA Laboratory Test in Maternal Blood by NIPT Method for Trisomy 21 Diagnosis

الملخص

There are many ways to diagnose Down syndrome during pregnancy. The existing methods are not without risk, but cause abortion or are associated with labor pain. The purpose of this research is to compare the amino synthesis test and the test (NIPT) which has benefits for the diagnosis of Down syndrome during pregnancy. The test (NIPT) is a non-invasive prenatal test that analyzes free fetal DNA in the mother's bloodstream. A new method for separation and testing during pregnancy for various trisomy’s, especially trisomy 21, is used in the case of the fetus. Using this method in singleton pregnancies with a high probability of trisomy 21 for reasons such as the mother's old age, abnormal serum isolation, Personal or family history of aneuploidy and abnormal solography has been confirmed. Using the NIPT method is a non-invasive test. And the pregnancy is not exposed to side effects as well as miscarriages associated with invasive testing methods. Using this method, at least 99% of trisomy 21 pregnancies can be detected and identified. After performing this test, one case out of 1000 cases of trisomy has a normal answer, which is lost in isolation. Positive answers for trisomy 21 should be investigated and confirmed through invasive laboratory methods. In the new NIPT method, which has advantages, this method can be used in a wide range of fetal disorders among the uses of this method, we can mention monogenic disorders. In this research, the library method and scientific research articles have been used.